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From the gene list we selected genes not significantly changed between the tissue groups.
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HA intensity in the cancer associated stroma was not different between the three tissue groups examined (P = 0.443, Pearson Chi Square).
Again, differences in levels of proliferation between the two tissue groups remained significant with elevated cell cycle entry in cultures derived from peripheral regions (Figure 2F).
Analysis of variance testing between the different tissue groups.
There were significant correlations between the major tissue groups (lean, fat and bone).
Here, we use fold change (FC) defined as FC = ExpressionBrain/ExpressionReference to quantify expression differences between the two tissue groups.
The comparison of the distribution of relative expression values between both tissue groups showed only a small overlap.
We performed Gene Set Enrichment Analysis (GSEA) and Expression Analysis Systematic Explorer (EASE) pathway analysis of the genes lists obtained from all of the comparisons between tumor and tissue groups.
To establish differences in gene expression between tissue groups, both gene-by-gene statistical testing using the permutation test with t statistics and singular value decomposition (SVD) of the data matrix were employed.
Comparison of methylation between tissue groups, gestational ages and RE evolutionary age was performed using the non-parametric ANOVA, Kruskal-Wallis test, followed by Dunn's multiple comparison tests.
There was no significant difference between the tumor and adjacent tissue groups.
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