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Unfortunately, all three tested K. oxytoca strains (AHC-6, Mut89 and Mut89 + npsB) inhibited epithelial wound healing significantly (p < 0.001), showing no differences between the three strains (p = 1) (Figure 5b, c).
Interestingly, most of the proteins subject to phase variation between the three strains are related to virulence.
Interestingly, no marked differences were observed in the neuraminidase inhibition tests, i.e. the IC50 between the three strains (Fig. 9A).
As expected, the differences in number of ORFs between the three strains were much smaller when the same methods were applied for ORF prediction (data not shown).
The separation between the three groups was mostly along PLS1, suggesting that this PLS component contains metabolites associated with differences between the three strains.
Other compounds discriminating between the three strains were creatine, creatinine, allantoin, and hippurate, all of which increased in the mutant mice.
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The diagram shows the number of shared and unique clusters between the three strain genomes.
The difference observed here between the two strains only became apparent after 7 h of culture.
In contrast, the carboxymethylcellulose (CMCase) activity showed no obvious differences between the two strains (Fig. 3f).
Beta3 subunit mRNA and protein expression levels did not differ between the two strains.
Sequencing PB2 revealed that only a single base pair differed between the two strains.
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between the three bacteria
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between the three novels
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