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After correcting for control mortality, the sterility of A. ferus adults ranged from 90.5 to 99.1% at 20 Gy and between 99.0 and 99.6% between the three replicates at treatment doses of 40 to 100 Gy.
Samples were excluded from the analysis if the coefficient of variation between the three replicates was <0.1.
The Spearman's correlation coefficients (Rs), and the coefficient of variation (CV) between the three replicates was calculated using the 309 common microRNAs.
Concordance between the three replicates was strongly correlated with microarray signal strength, ranging from virtually 100% for the most strongly expressed genes to almost 0% for the most weakly expressed genes.
The number of MS2 spectra was also reproducible between the three replicates of each method.
The correlation between the three replicates was very good, with an interclass correlation coefficient of 0.97.
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The possible differences between the three replicate experiments were modeled as a random block effect.
This procedure was done to mitigate the effect of potential unexplained variation between the three replicate measurements on the 'Phage effect' variable.
For performing one-way ANOVA test, normality and equal variance must be considered; the difference between the five replicates must be insignificant and the model designed by the software should be reliable.
There was considerable variation in rhodamine concentrations following injection between the six replicates during the first 36 h but very uniform levels thereafter, and the equilibrium level was reached after 84 h (Fig. 8b).
No significant differences were detected between the two replicates groups for the same treatment (at egg, fry or juvenile stages).
More suggestions(16)
between the three areas
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between the three positions
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