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Exact(6)
By looking at the transitions between the three maps, an overall increase in Gini coefficients is expected.
After their removal the remaining corresponding markers are in close agreement between the three maps but still reveal inconsistencies with the sequence assembly.
Based on the number of shared and specific recombination coldspots and hotspots (Venn diagram in Additional file 10), we calculated a Jaccard index to assess the similarity between the three maps (three pair-wise comparisons).
We used the Wilcoxon signed rank test to test the hypothesis that 'map lengths are equal between the three maps: G2F, G2M and F2.' This hypothesis was not rejected for the comparison between G2F and G2M, P-value (G2F versus G2M) = 0.78, indicating that sex had no significant effect on map length in this mapping population.
By aligning homologous linkage groups, we were able to compare the numbers and locations of recombination coldspots and hotspots between the three maps obtained for the different genotypes (two intraprovenance hybrids for the G2 population and one interprovenance hybrid for the F2 population).
The exception was 54 markers of the E map in which the SNPs used to label a specific bin were selected among those in common with T×E and T1E to facilitate visualization of the map comparison presented in Figure 2. The SSRs were all added as markers in the maps so they could be used as anchor points between the three maps studied and with published Prunus maps.
Similar(54)
The tests described in Methods revealed a number of differences between the three mapping applications.
However, after some time, the similarity between the two maps decreased.
Common genetic loci were used to define syntenic regions between the two maps at a macroscopic scale.
Let us assume that there is a significant offset between the two maps, and that one of the parallel roads needs to be matched.
National maps were produced in different years, and thus some differences between any pair of maps would be caused by intervening growth and disturbance in the period between the two maps.
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