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The similarities between the three assemblies (90, 98 and 90e) are illustrated in Figure 1 a Venn diagram which shows that 72.68% of the raw sequencing reads were integrated into contigs common to all three assemblies, and 20.51% of the sequencing reads make up a shared pool of single sequencing reads (singletons).
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ChrX is the chromosome showing the largest difference between the two assemblies, with strikingly fewer assembled sequences in Btau than UMD (88.5 Mbp versus 148.8 Mbp, respectively).
Surprisingly, only 448 contigs are identical between the two assemblies based upon results of a BLASTN alignment [30].
For the current work, our focus was on the differences between the two assemblies.
The gene conversion characteristics were indistinguishable between the two assemblies (data not shown).
First, we used MUMMER Nucmer [ 64] to detect orthologous sequences between the two assemblies.
The average proportion of heterozygous genotypes per marker was comparable between the two assemblies except for chromosomes 2A, 3D, and 4A, where differences between the two assemblies were large.
The divergence between the two assemblies is also reflected by the number, composition and organization of the gene segments.
The Net alignments were converted into PSL and BED formats to facilitate analysis of the differences between the two assemblies.
The most obvious difference between the two assemblies is that Btau_4.0 unplaced contigs are placed on UMD3.
There are 2 single nucleotide differences between the two assemblies mapping to IFNA17 and one dinucleotide difference.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com