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The large number of positions not identically aligned between the three alignments made us confident that the alignment space investigated was not too narrow.
Cross-comparison between the three alignments also revealed that some short (about 2 10 bp) stretches of bases in a few sequences in two of the alignments were likely to be misaligned due to incomplete sequencing resulting in long leading or trailing gaps in these sequences; these stretches were coded as missing data.
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Only 120 of the 33.6 million (0.0003 %) reads were aligned differently between the two alignments.
To emphasize, overlapping denotes some coverage of common sequence ranges between the two alignments for the same domain model.
When the distance between two alignments on the same chromosome was short, the genomic sequence was further analyzed by dot plot to identify any potential continuity between the two alignments.
The second is a Genomic Distance Penalty (GDP) calculated as log10 of the number of base pairs along the reference genome between the two alignments.
All invertebrate-type lysozymes could be reliably aligned to each other at both protein (alignment 2; Fig. 3) and DNA sequence level (alignment 3; position of indels is identical between the two alignments).
Although we report results across the full spectrum of alleles, the proportion of sites that were significantly biased in both the alternate and reference alignments was almost the same regardless of the absolute concordance between the two alignments.
Additionally, there may be overlap between the two alignments (V) because bases at the breakpoint in the read could be assigned to either location in the reference genome without changing the junction sequence.
Blast analysis of the first 764 nucleotides of the reverse complemented GenBank: FJ985864 indicated that nucleotides 1 359 aligned perfectly, beginning 26,374 nucleotides downstream in the same BAC, and nucleotides 403 764 aligned perfectly, beginning 35,659 nucleotides downstream also in the same BAC, with a gap of approximately 8900 bp between the two alignments (data not shown).
We found a good agreement between the two alignment methods in the seven experiments where both blood vessels and electroporated neurons were used.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com