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The proportion between the solid medium and gaseous culture was always 1 5.
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They are propagated when the solid medium near the surface has varying vertical elastic properties.
The solid medium contained 1.5% agar.
The solid medium contained Bacto-agar (1.5%).
The solid medium was prepared by the addition of 2% agar into the liquid medium.
Two or three colonies from the solid medium plate used as inoculum were transferred into culture medium.
Seeds were placed against the cut agar surface and allowed to germinate into the solid medium.
A loophole of cells from the solid medium was placed into 500 mL of medium without agar.
When required, the solid medium was obtained by adding 15 g/l of Difco agar.
A total of 20 g/L of ultrapure agarose was added for the solid medium.
Hairy root selection started 1 week after roots were transferred to the solid medium.
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