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However, the differentiation among the four groups of smaller poodles, defined on the basis of color and size, is about four times larger than the largest difference observed between the size classes for the three small-sized poodles, reaching FST values of up to 0.120 (Table 1).
Nevertheless, only results for PM2.5 are reported in detail because of the high correlation between the size classes.
Differences were apparent in the make-up of genes contributing to this GO category between the size classes.
Hence, while the expression of individual genes demonstrated relationships with body size, the overlapping nature of their assignments within GO categories restricted the detection of significant differences between the size classes examined.
The patterns of genetic exchange suggested by the pedigree closely parallel the genetic differentiation between the size classes (Table 1), as confirmed by a Mantel correlation test (Z = 0.650, p = 0.039).
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These early and later stages are strikingly different: only 19 proteins were found in common between the two data sets for proteins in the size classes between 3.5 and 20 kD.
To corroborate that the genetic differentiation observed between the poodle size classes is inversely related to the current gene flow between them, we examined pedigree information from the Swedish Kennel Club [ 24].
Distinguishing between those size classes allows one to identify size-dependent strengths and weaknesses of the tools considered.
The one thousand datasets were evenly distributed between the molecular size classes defined in the Methods section, as well as two selection methods: random draw vs. deliberately selecting diverse molecules (as implemented in the RDKit Diversity Picker tool in RDKit for KNIME [35]).
The nature of the loci identified was distinctly different between the two size classes: retrotransposons, and in particular LTR retrotransposons, accounted for the majority of 24 nt loci.
Using eight replicate survivorship trials, Johnsson (1993) found no significant difference in the distribution of risk between the two size classes (Wilcoxon signed-rank test: T+ = 29, p = 0.15).
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