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Individual heterozygosity (the proportion of heterozygous loci) was calculated for all individuals and Spearman's rank correlation between the sampling year and individual heterozygosity was estimated.
In Figure 1B, the time intervals between the sampling year of each CSD set and the assumed peak year 1970 are labeled Δ t1 to Δ t4.
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In the BB population, the number of PA increased from zero to eight between the sampling years (Table 1).
Time difference in years between the median sampling years between each temporal group was calculated.
Although the aflatoxin results are similar for both years, those of OTA and FB1 are significantly different between each sampling year.
In order to test for significance of these results, grade data for all students from the 2013/2014 year (which is representative of the average GPA of the past 5 years) were compared to data for the 2014/2015 year using an independent samples t test, which showed a significant difference in the average GPAs between the two sample years (p < 0.001).
The visualisation of the whole tree regression model (Fig. 5) clearly shows the good fit of the allometric model, spread of points across the range of diameter measurements and between the two sample years.
There were no significant differences (Two-Way ANOSIM; depth nested within sampling year) between sampling years.
The pairwise genetic distances (F ST) between sampling years were significantly correlated with the temporal difference between samples (Mantel's test, r = 0.647, P < 0.001; Figure 4), i.e. samples that were fewer years apart were more similar genetically.
Furthermore, the secular trends in reported sexual behaviour over the 30 year study period were so pronounced that declining response rates could not explain the differences between the samples of 70 year olds.
The occurrence of these yeasts varied substantially between sampling years, with most pronounced fluctuation being observed for M. reukaufii.
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