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Fold change was calculated as the mean ratio between the relative transcript levels.
Statistical significance of differences in the expression of individual genes was determined by using a Student's t-test between the relative transcript values derived from the dsHSC70B-injected and dsβ-gal injected mosquitoes across 3 replicates for each gene.
To identify genes that may play important roles in wheat defense against Hessian fly, we examined the ratio between the relative transcript abundance in resistant plants during incompatible interactions and those in susceptible plants during compatible interactions (defined as incompatible/compatible ratio, I/C ratio) (Additional file 4: Figure S1).
The ratios between the relative transcript abundance of the regenerant line and the other two lines showed that the markers tended to be under-expressed in the proliferating calli of the regenerant line (from 0.01 to 0.4) and over-expressed in the calli induced in embryogenesis on EXP medium (from 2 to 43 times).
The ratios between the relative transcript abundance of the regenerant and non-embryogenic lines (R/NE), the regenerant and embryogenic lines (R/E), of the normal and abnormal embryos (Nor/Ab) are presented for only significant down-regulated genes (< 1) and up-regulated (> 1).
In Table 2, the level of expression was calculated as the ratio between the relative transcript abundances in callus of the regenerant line/embryogenic line, the regenerant line/non-embryogenic line grown on ENT and EXP media, and in normal/abnormal somatic embryos.
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In all three datasets, there was no difference in the relative transcript abundance between mRNA from group II and total mRNAs (P > 0.05).
The Y axes indicate the relative transcript levels of each gene.
The relative transcript abundance of all the OsNCX genes under various abiotic stresses viz.
The Tubulin transcript was used as an internal control to quantify the relative transcript levels.
The relative transcript levels were expressed as the mean ± s.d.
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between the relative core
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