By comparing analysis of eyes, skin and peritoneum, we conclude that the regulation pattern is similar between these different tissues in this species which is relevant for the cryptic life strategy of this species.
Moreover, none of these genes depicted the regulation pattern observed in the thyroid.
When comparing the regulation pattern of the three MAP kinase subgroups, no uniform pattern was discerned between tissues during torpor.
Clustering algorithms measure the similarity between observed gene regulation patterns across the various conditions, and assemble clusters such that similarly regulated genes are grouped together.
We next examined the relationship between gene regulation patterns and uterotrophic effects of E2 versus E4, noting histological changes and uterine epithelial cell proliferation.
It is notable that in most cases, the gene regulation patterns were the same between Luminal A and Luminal B subtype samples, as only 9 genes displayed different regulation between these 2 subtypes.
The few orthologous genes that show the same regulation pattern between the two species may be conserved, but the small number may be related to the phylogenetic distance and the different growth conditions that allow yeast or mycelium to develop.
More strikingly, several genes were oppositely regulated, including 19 genes down-regulated in p8+/+ but up-regulated in p8-/ liver and one gene up-regulated in p8+/+ but down-regulated in p8-/ liver, which underscores the differences between LPS-induced gene regulation patterns in p8+/+ and p8-/ livers.
On the other, Z-score analysis asseses the match between observed and predicted up/down regulation patterns allowing for Bayesian scoring of the results [ 27].
A direct comparison of the gene regulation pattern between expressing and control strain at the lowest oxygen concentration (8%), in contrast, revealed only six genes (see Table 3) to be differently expressed (adjusted p-value ≤ 0.05).
