Sentence examples for between the reference gene from inspiring English sources

No more than 10% difference in primer efficiency between the reference gene and the target genes was obtained.

ΔΔCT is the difference in CT-value between the target gene and the endogenous control minus the difference in CT-values between the reference gene and the endogenous control.

We next calculated the relative evolutionary rate of each single sequence pair by dividing the distance between the two sequences of a viral gene by the distance between the reference gene sequences for the same pair of viruses.

By definition, Ct values equal the required number of amplification cycles to reach the threshold fluorescence level (automatically determined); Δ Ct implies the difference in the Ct values between the reference gene (mean) and the gene of interest (CDK19 or ELOVL5) and ΔΔ Ct represents the difference between two Δ Ct values obtained from patient and the control (mean).

With a minimum threshold of 60% aa similarity between the reference gene and the target ortholog, 1063 ORFs (96%) were accepted automatically by GATU and a further 24 were accepted from the reference genome after reviewing data in the Unassigned ORF table.

The main difference between the reference genes and the four sets of disease-related genes is a difference in number of zero- or low STR content.

We controlled for this by only selecting fusion candidates with 13 bases or less of sequence in common between the reference genes (Additional file 1: Figure S2).

It could also be done by viewing the ΔCt between the reference genes across all samples (as illustrated in Fig. 1), a method that should result in a very small standard deviation.

The pre-publication history for this paper can be accessed here: CEP 17 = mean/cell of the chromosome 17 centromere probe signals HER-2 = mean/cell of the HER-2/neu probe signals FISH Ratio = the ratio between HER-2 and CEP 17 Real-Time PCR Ratio = the normalized ratio between HER-2 and the reference gene (gastrin) "+" = positive, "- =negative.

When the relative differences in threshold cycle between the viral genes and the reference gene (ΔCT) and expression relative to controls (ΔΔCT) were calculated, they were transformed by the equation 2−ΔΔCT 53.

This method calculates the probability that the association between experimental gene set and the reference gene set associated with a canonical pathway is due to random chance.