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Spectral comparison between the reference dye C153 and all our Mn-doped NCs. Figure S2.
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It is expected that TICR would show better photovoltaic properties than the other dyes, including the reference dye.
In contrast, device based on the reference dye reaches 5.22% at most.
Comparative measurements were taken using non-doped nanoparticles that have been mixed with the reference dye.
Fluorescein was added at a final concentration of 10 nM as the reference dye.
To avoid oxygen cross-sensitivity of the reference dye, we incorporated the dye in virtually gas-impermeable PViCl−PAN nanoparticles.
All expression data was normalized to β-actin and corrected using the reference dye ROX.
Intensity ratios IR are calculated for each individual well by dividing the intensity of the indicator dye by the intensity of the reference dye.
The reactions contained the passive reference dye, ROX.
The ROX reference dye was present in the RT-qPCR reaction buffer.
The passive reference dye, ROX, does not participate in the amplification reaction but provides an internal reference to which the reporter-dye signal can be normalized during data analysis.
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