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In addition, highly confident functional predictions for 6 of these ORFans were derived by analyzing the conservation of functional motifs between the predicted structures and proteins of known function.
Finally, notice that the biases, which give the distances between the predicted structures and the reference structures, are more than twice as large as the SDs of the distances of ensemble members around the predicted structures, which shows that the predictions are substantially more precise than they are accurate.
The exact molecular mechanisms regulated by this complex remain largely unknown but there are marked similarities between the predicted structures of the BBSome components and those of the subunits of the COPII and clathrin cages, indicating a possible function of the BBSome as a coat complex [11].
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In our study, two types of root mean square deviations (RMSDs) between the predicted structure and the corresponding crystal structure, including ligand RMSD (L_RMSD) and interface RMSD (I_RMSD), were used as the criteria to evaluate the performance of protein protein docking.
When parts of the structure are forced using constraints, we find that the FE difference between the predicted structure of RNase P and the correct structure is only 3 kcal/mol.
Surprisingly, the RMSD between the predicted structure of the ZIP10 PL domain and the PrP and Dpl structures returned even lower values of 2.6 Å and 2.9 Å, respectively, indicating that the primary structure of the ZIP10 PL domain is highly compatible with the basic prion protein fold.
In this context, it is particularly exciting to note that Kelman and colleagues comment on a potential similarity between the predicted structure of eukaryotic Cdc45 and RecJ [ 6].
To determine whether altered structure contributed to a large delta SEN, we examined the delta SEN as a function of base-pair distance between the predicted structure for the subalignment and the given structure of full alignment and found no strong correlation.
In contrast, the predicted structures of genes that are homologous to known sequences were largely congruent between the orthologous BACs.
These discordances could be related to the differences found between both models in the predicted structure for this region of the protein (Figure S3).
The predicted structure of the OB domain of Obelix appears very similar to that of eIF1A, but contains an extra sheet structure between sheets β3 and β4.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com