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A 10 bp overlap between the partner sequences was allowed at the fusion point to account for alignment uncertainties.
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The crossover sites were identified as located between the last nucleotide, differentiating the clinical sequence from the 3' partner reference sequences, and the first nucleotide, differentiating the clinical sequence from the 5' partner reference sequence.
For linkage, we required monophyletic clustering between enrolled partners' sequences and a Bayesian posterior probability of ≥50%.
This second phase of sequencing did not reveal phylogenetic linkage between the partners in any of these pairs, again suggesting that superinfection was not occurring.
Considering that biological interactions do not operate based on sequence homology between partners, sequence homology-based methods such as Basic Local Alignment Search Tool BLASTT) [ 5] are generally not useful for detecting interacting modules.
There were other differences between the partners.
Mario the celebrity chef produced strains between the partners.
Profits are split evenly between the partners.
Few families have perfect equality of incomes between the partners.
Moreover, a Nash-equilibrium method is considered between the partners of the chain.
Relational work consists of sustained reciprocal efforts between the partners".But reciprocity doesn't mean equality.
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