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Surprisingly, resveratrol opposed 947 (92%) of age-related changes in gene expression, and 522 of these represented highly significant differences in expression between the old control and old resveratrol groups (P≤0.01).
As previously reported [2], we observed a large effect of CR in opposing age-related changes: CR reduced 921 (90%) age-related alterations in gene expression and 536 of these represented highly significant differences (P≤0.01) in expression between the old control and old CR groups (Figure 1A).
There were no significant differences in the IL-4 production of splenic lymphocyte stimulated by Con A between the old control mice and the young mice without treatment.
‡ Fold change calculated by EdgeR from pairwise comparisons between the young and old control groups for the age effect, or between the old control and old CR groups for the CR effect.
We performed pairwise comparisons between the young and old control groups to measure differences in the circulating miRNAs associated with old age, and pairwise comparisons between the old control and old CR groups, to unravel any potential effect of CR on the age-associated changes in circulating levels of miRNAs.
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The difference between the old controls and old ischemics was also significant (p = 0.049), with an average difference of 5.4 cycles.
The old control the young; the men control the women.
Notably, there was no difference in the degree of whole body insulin resistance or any other baseline parameter between the Old-Control and Old + GLP-1 groups prior to GLP-1 infusion (Table 1).
Although the older control group reduces its employment rate more strongly, the relatively large gap between the employment rate of the displaced and controls is persistent.
Greater differences were found between the torn groups and the subscapularis (old) control groups, with torn tendons exhibiting significantly thinner fibrils (Fig. 4), lower ratios of thick-to-thin fibres (Fig. 5), and shorter crimp lengths (Fig. 6) compared with the subscapularis (old) controls.
We performed pairwise comparisons between young and old control groups to measure the differential abundance in circulating 5′ tRNA halves associated with old age, and between old control and old CR groups to determine whether CR has an effect on any age-associated changes.
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