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The presence of a coverslip between the objective lens and the tumor surface may be sufficiently flattening the surface to be in line with the optical section.
The temperature of specimen should be kept below devitrification while the distance between the objective lens and specimen should be short enough for high resolution imaging.
The pre-objective configuration is effective for a long working distance between the objective lens whereas the post-objective has no off-axis aberration with relatively high image resolution.
The imaging system operates under control of SlideBook 4.2 (Intelligent Imaging Innovations Inc, Denver, CO) and includes a computer controlled spherical aberration correction device (SAC, Intelligent Imaging Innovations, Inc, Denvers, CO) installed between the objective lens and the back illuminated CCD camera (Cascade 512B; Roper Scientific, Photometrics, Tuscon, AZ).
The spacer bars maintained a constant distance between the objective lens and the tissue to be imaged.
For an altered distance A between the objective lens and the sample surface, the focusing depths B1, B2, and B3 of the blue, green and red beams, respectively, were derived by TraceProTM modeling, as illustrated in Fig. 3.
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A dry objective is one that works with the air between the specimen and the objective lens.
Because of the strong field between the sample and the objective lens, even a small tilt of the sample generates a parasitic dipole field, which decreases resolution and causes image deformations.
However, in some cases, like in the cathode lens between the sample and the objective lens in the photoemission electron microscope, even a small imperfection can impair the resolution significantly.
The shape of the PSF for a specific bright-field microscopy setup is dependent on the NA of the objective used, the refractive index of the medium between the sample and the objective lens and the wavelength, λ, of the image forming light 6.
This exact tuning is accomplished by variable magnification between the micro-mirrors and the objective lens using an additional transfer lens.
More suggestions(15)
between the acoustic lens
between the objective theme
between the chromatic lens
between the objective quality
between the scan lens
between the red lens
between the objective reduction
between the last lens
between the objective truth
between the intraocular lens
between the objective body
between the objective response
between the main lens
between the thermal lens
between the objective reality
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