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This phenotype was haploinsufficient, since the heterozygous mutant exhibited an intermediate resistance between the null mutant and the wild type.
The second category of genes found to be differentially expressed between the null mutant AMC and the other alleles concerned the development of the sensory organs and more particularly the larval olfactory system.
Moreover, there is no significant correlation between the null mutant fitness for a given HI gene, and the fraction of species in which its orthologues are retained (correlation coefficient = 0.06).
In these conditions, the dynamics of EAG responses clearly differed between the null mutant Est-6°, which completely lacks Est-6, and the two control strains, that is, the wild-type strain Canton S (' CS') and the rescue strain (' Rescue'), in which Est-6 expression was restored: the depolarization was similar in the three strains but the repolarization was slower in Est-6° males.
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The null mutant of NS5 MTase is lethal for virus.
In addition, we distinguished between embryos expressing PTBP1 from the null mutants using immunofluorescence on cryosections.
In addition, we noticed that the CD kinetics differ between parasites expressing CPC and the null mutants.
The null mutants thus obtained were designated as (−/−GLOI+).
All the phosphatase null mutants produce comparable gliotoxin levels than the wild-type, except for the null mutants for DspA, PtcD, and PpzA that were able to produce between 1.5- and 2.4-fold more gliotoxin than the wild-type strain.
We found the null mutants of EMC1 and EMC7/8 show the phenotype which is characteristic to the dPob null mutant.
Each value corresponds to the difference in the feeding rates between the triple null mutant and the ser-7 null mutant presented in (C ) and (D ).
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