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In the colorectum, CFM detected architectural differences between the normal crypt structure and neoplasia [ 11, 12].
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In sections that captured adenomatous structures adjacent to the normal crypt epithelium, a distinct boundary between the fascin-positive adenomatous area and the fascin-negative, normal epithelium was seen (Fig. 1g).
Thus, the normal crypt can efficiently eliminate β-catenin mutant cells, whereas APC mutations favor retention.
In the normal crypt, expression was concentrated around the base (Fig. 1G).
Notably, the lack of Id2 increased the number of apoptotic cells in the normal crypt epithelium of the mice.
SMARCC1 showed weak staining in the basal half of the normal crypts in 9 of 10 specimens.
Notably, in some implantation sites, while there are also branched crypts, the embryo ended up in the relatively normal crypt and established an axis that is similar to the normal condition (Supplementary information, Figure S5).
Under light microscopy, a scalpel blade was used to excise the ACF of interest and the surrounding normal crypts.
The stained crypts exhibited atypical nuclear organization, longer and larger mucosal lining, marked condensation of the nuclear materials and narrower lumen compared to the surrounding normal crypts.
Furthermore, there was no difference in the percentage of Ki67 positive cells in normal crypt epithelium between ApcΔ716 and Apc Id2 mice.
In micrometastases, myofibroblasts are found intimately surrounding glandular structures in the same way that they surround the normal colonic crypt.
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