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The two studies differ in the degree of divergence tolerated between the native gene and its heterologous replacement.
However, the bias of codon usage between the native gene sequence and P. pastoris has significant impact on the expression level of recombinant protein.
Similarly, significant differences between the native gene set and that of the xenologues were noted for GC composition and the selection pressure existing on the genes compared.
It has now been shown that the difference of codon usage between the native gene sequence and expression host has significant impact on the expression level of recombinant protein [ 14, 15].
Due to the difference of codon usage between the native gene sequence and expression host, researchers have used codon optimization to increase the expression level of heterologous genes in P. pastoris.
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Relative neutrality plots differed between the native genes and the putative xenologues.
Finally, the correct nucleotide sequences of the native gene and all mutated genes were verified.
Finally, the correct nucleotide sequences of the native gene and all truncated genes were verified.
The most parsimonious explanation for the discrepancy between native GSTF8 expression and the expression of the GSTF8 LUC reporter gene is that the isolated GSTF8 promoter sequence used in the GSTF8 LUC gene construct, which corresponds with the short promoter as defined by Thatcher et al. [ 34], could contain cis-acting suppressor elements that are masked in the context of the native gene.
The new modified IFN-CSP gene has the identical amino acid sequence as the native gene.
Irrespective of this, our results indicate that despite binding of HIF-2 α to the native PHD3 HRE in the first intron, there is a marked difference between the activity of HIF-2 α in promoting transcriptional activity mediated by this sequence, and its activity in promoting expression of the native gene.
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