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We found a positive relation between the morphological score and the fertilization success rate.
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For the morphological score, the degree of fragmentation of the embryo and the uniformity of the blastomeres were assessed daily.
The morphological scores on Day 5 showed a similar trend.
The analysis of the morphological scores was performed using the Mann–Whitney test.
The means ± SEs of the morphological scores were determined for the four independent experiments.
To assess the morphological scoring criteria (vitelline circulation, flexion, heart, brain, gross facial deformities, and limbs) between exposure groups, we used Kruskal-Wallis one-way analysis of variance (ANOVA) followed by Mann-Whitney U-test for nonparametric data.
In the light of the backcross hypothesis, some of the anomalies arising from the morphological scoring system can be revisited.
There was no significant association between male age and the average morphological score, with a mean decrease of 0.02 [95% CI 0.002 to −0.044] (P = 0.07).
Logistic regression analysis was performed to assess the association between number of cells on day two and three, morphological score day two and three, ICM score, trophectoderm score and expansion status with the success of establishing a new hESC line.
Complete information on the number of cells and morphological score on days two and three, ICM score, trophectoderm score and expansion status was available for 206 embryos.
The %INUC scores indicate a clear-cut discrepancy between the morphological diversity observed in neuropterid orders and the low rate of substitution that characterizes the evolution of their codon families.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com