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The DAVID tool was developed for GO-ranking, and is independent of methodological differences between the microarray analyses tools used in this study.
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The results represent the mean ± standard deviation of the relative expression in qPCR of six differentially expressed genes identified in the microarray analyses between Se and Sa challenges.
To test the hypothesis and to confirm the results of the microarray analyses, the link between HSF2 and pathways facilitating invasive properties, that is, GTPase activity and actin cytoskeleton signaling, was explored.
For the microarray analyses, expression differences between the groups where assessed by Student's t-test; p < 0.05, and data are presented as log2ER ± SEM.
Differentially expressed genes in the microarray analyses.
Candidate sequences were extracted from the 50 most highly upregulated genes from the microarray analyses.
GPM1 and TDH3 were selected as reference genes based on data obtained from the microarray analyses where both genes were shown to have high expression values and minimal variation between the different treatments.
The microarray analyses were performed in duplicate.
Mårten Sundberg provided technical assistance for the microarray analyses.
MB: Performed the microarray analyses.
SK supervised the microarray analyses.
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