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Comparison between the mean quantities obtained from the SRM and these measurements show very good agreement.
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All reactions were run in duplicates with the mean quantity taken.
The mean quantity recovered in dialysate was 135.7 ± 8.9 mg.
For each sample, the mean quantity of each target gene was normalized to the mean quantity of B2M.
The r.s.d. of the mean quantity of milk consumed per meal was high at all ages indicating an important variability of the trait between animals and meals.
In each sample, the mean quantity of each miRNA was normalised to the mean quantity for the endogenous controls RNU48 and RNU6B, according to the following formula: miRNA expression=candidate miRNA expression mean quantity/ RNU48 mean quantity+RNU6B mean quantity)/2).
The mean quantity of each gene was divided by the mean quantity of ACTB and was used for the normalization of input DNA.
The mean quantity of genes was divided by the mean quantity of ACTB and was used for the normalization of input DNA.
For each gene the mean quantity was calculated from triplicates for each sample, and this quantity was normalized to the similarly measured mean quantity of the glyceraldehyde-3-phosphate dehydrogenase normalization gene.
For breast tumour Quantitative Real-time RT-PCR data, a two-sample t-test allowing for difference in variance between the two samples was used to test the difference between the mean standardised quantity of RNA for the ER-positive and ER-negative groups.
Lumped values of near-surface and deep soil water content are then disaggregated at local scale using simple relationship between mean quantities, local topography and soil depth information.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com