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Growth curves of these L. braziliensis isolates were performed and no statistically significant differences were observed between the isolates used for macrophage infection.
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We used all unique SNV positions for calculating distance between the isolates using pair wise alignment.
At this point in time VI101 had significantly (p < 0.05) lower numbers of intracellular bacteria compared to the other isolates except for Mah H1, while no significant difference were observed between the other isolates using the paired t-test.
However, a major difference between the two MHV isolates and the two IBV viruses we used was that the two MHVs were pathogenic but demonstrated different pathogenicities, neurovirulence and hepatitis, respectively, whereas the difference between the two IBV isolates used in this work was that one causes disease and the other does not.
The relationship between the F. verticillioides isolates used in each location is unknown; as such, it is possible that differences in pathogen aggressiveness could have contributed to the disparity in mean ear rot values across environments.
In order to look for commonalities between LF82 and the IBD isolates used in this study, we compared the inferred genomic sequence of our IBD strains with that of LF82.
Single nucleotide polymorphisms (SNPs) were identified between the sequenced isolates using NUCMER and dot plots were generated using MUMmerplot [ 72].
Considering the diverse phenotypes of different accession-isolate combinations, the observed phenotypic variation between the two studies is likely due to the PPV isolates used.
We observed differences between the eight HIV-1 isolates used in the present study.
The differences in transcriptional profiles could be explained by several factors including experimental conditions, type of array technology and intrinsic differenced between isolates used in all three studies.
The only difference between the HpSVd-hop and -grapevine isolates used for the inoculum was at nucleotide 54, which in HpSVd-hop was adenine (A) and in HpSVd-grapevine guanine (G) [47].
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