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In addition, the intermediate capillary plexus, between the inner plexiform layer and the inner nuclear layer, is denoted by green.
Further AMOTL1-lacZ expression pattern characterization revealed staining in the adult retina, extending from the inner limiting membrane (ILM) to the junction between the inner plexiform layer (IPL) and the inner nuclear layer (INL).
In addition, two capillary branches of the intermediate plexus and the deeper plexus are located in between the inner plexiform layer and the inner nuclear layer as well as between the outer plexiform layer and the outer nuclear layer, respectively [ 13].
Actually, only three layer borders with falling contrast should lie in the ISG beneath the ILM, namely: the ONFL; the border between the inner plexiform layer and the inner nuclear layer; the border between the outer plexiform layer and the outer nuclear layer.
W3-RGCs were identified by their characteristic morphology (Kim et al., 2010 ; Zhang et al., 2012 ) with small dendritic fields (territory size: 10,783 ± 409 μm, n = 25) filled by densely branched neurites stratifying in the center of the IPL with a secondary arborization near the border between the inner plexiform and inner nuclear layers (INLs).
Subsequently, the stack of flattened retinal images was rendered in 3D and a sub-volume was obtained from locations corresponding to the interface between the inner plexiform and inner nuclear layers, and the outer plexiform layer of the retina, to visualize the inner retina microvasculature.
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Since the newborn rat ganglion cells are stacked in a pseudostratified fashion and it is difficult to detect the border between the RGC layer and the inner plexiform layer [16], we cannot exclude the possibility to have counted pyknotic profiles in both of these layers with the cresyl violet staining procedure.
Due to the low contrast between the ganglion cell layer and the inner plexiform layer both were combined and evaluated as one GCIP.
Additionally, the cells infiltrating the vitreous had abnormally shaped nuclei, and evidence of apoptosis was found in the retina between the intra-lamellar membrane and the inner plexiform layer.
The average thickness of GCC (between the internal limiting membrane and the interface of the inner plexiform layer and the inner nuclear layer) was measured.
The correlation between the level at which a bipolar axon arborizes in the inner plexiform layer and its physiological response to light is very strong; ON-bipolars arborize in the inner half of the IPL whereas OFF-bipolars arborize in the outer half of the IPL.
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