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We did so, since RQ values provide a more accurate comparison between the initial amounts of template in each sample, without requiring an exact copy number for analysis.
In order to test the hypothesis that increasing CT values magnifies the natural variation between the initial amounts of samples loaded in each well during RT-PCR, we examined the standard deviation of the genes against their mean CT values, as shown in Figure 1.
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In this case, the life span of the cultivated cells is governed by the balance between the initial amount and the consumption rate of glucose.
For samples with successful amplification there was a good correlation between the initial amount of soybean in the mixture and the output from PCR, when suitable block designs were used to control experimental errors.
The amount of PAHs sorbed to the soil was obtained through calculating the difference between the initial amount and remaining in the solution.
The drug entrapment efficiency was expressed as percentage of the MMC difference between the initial amount of MMC and the free amount in the supernatant relative to the total amount used for the nanoparticles preparation.
There is an exponential relationship between the initial amount of template DNA and the cycle threshold (CT) values.
The active fraction of PTX (X, bound to tubulin), was calculated as the difference between the initial amount (A) and the total of inactive drug (B, C, D) [X = A - (B + C + D)].
Negative correlation between the initial amount of genomic DNA in the template and the Ct (cycle threshold) value obtained after amplification reflects the concentration-dependent efficiency of real-time PCR reaction.
The amounts of intact enzyme were determined from density measurements on immunoblots and were compared to the initial amounts.
We can see this pump reversibility as an infinite oscillation between two values, the initial amount of potassium outside the cell, KO, and the final amount of substrates, after the forward running is complete and before the reverse running starts.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com