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After the mixture exposure, there were significant differences between the highest dose of mixture groups and the lower ones at the same time point, which do not conclude the death caused by mixture-exposed groups at 20 mg/L BPA from 48 hpf.
In addition, there was an overall main effect on the preference for ethanol over water at both time points [6hrs: F(3.10) = 6.9, P<0.01; 24hrs: F(3.10) = 4.2, P<0.05], however, post hoc analysis revealed a significant difference between the highest dose of ezlopitant (10 mg/kg) and vehicle only at the 6 hour time point (P<0.01, data not shown).
As used in this project, a biological threshold is the transition point (observed or expected) between the highest dose that will not elicit a given biological effect, and the lowest dose that will.
To generate a conventional power curve, calculations were made using a two-sided t-test (P < 0.05) under the assumption that the difference in average change from baseline values at end of study, between the highest dose and placebo was 1.75 with SDs of 6.23 and 5.98, respectively.
On the basis of interim data from a study in oocyte donors at risk of OHSS (Alvarez et al., 2007b), in which moderate/severe OHSS was reported for 24% (7/29) and 52% (13/25) in the dopamine agonist and placebo groups, respectively, 50 subjects per group were required for the present trial to show a significant difference between the highest dose of quinagolide and placebo.
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The fact that the separation by exposure dose is clearest between the lowest doses, with some overlap evident between the highest doses of 5 and 8 Gy has been reported previously in the literature [17].
In the random effects analysis, we saw no clear difference in the change in mean concentration of fasting blood glucose between the highest doses of GLP-1R agonists and controls (–0.51 mmol/L, 95% confidence interval –1.09 to 0.07; tau=1.06; χ test, P<0.01; Egger's test, P=0.487).
The maximum thickness of the implanted layer can range between 350 and 400 nm for the highest dose of 1×1018 cm−2.
In the case of the highest cell dose administration (50×106 cells), immune "conflict" might occur between the high dose of introduced MNC hUCB cells and the host immune system.
There were no significant differences between the high dose, low dose, and either dose groups vs. the placebo group in the primary clinical outcome measures.
On the other hand, another study reported no association between the high dose exposure to another estrogen disruptor, dioxin, and sexual maturity disorders in premenarcheal girls [ 53].
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