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Contrast filters differentiate between the gray values of objects of different colour but of similar brightness.
Results were expressed as the mean±s.e. of the optical density ratio between the gray values of isoprenylated and nonisoprenylated proteins for p21 rhoA and p21 ras, and between the nonphosphorylated and phosphorylated immunoblot bands for p42ERK2/MAPK.
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Moreover, the relationships between the gray value of the EL image and EQE or J SC were deduced and discussed.
As stated above, we are interested in a displacement field u that minimizes the following Lagrangian functional (we use an Eulerian reference frame for description of the displacement): (1) with (2) describing the matching of the gray values between the template and the deformed source image and (3) for the regularity of the underlying velocity field.
The gray values of each building fluctuate greatly.
where g c is the gray value of central pixel and g p are the gray values of its neighbors.
The gray values were converted to relative mineral density.
Mean gray values from negative controls were subtracted from the gray values of the immunostained sections to exclude background staining.
in which: J n is the gray values after treatment; Imax, Imin is the largest and lowest gray values in original image respectively; I n is the gray value before treatment; a is luminance compensation coefficient.
Average gray value was calculated within selected areas as the sum of the gray values of all the pixels in the selection divided by the number of pixels.
To quantify the difference of the fluorescence between N. meningitidis and NC, the gray value, an indication of the brightness of a pixel, of fluorescence images was processed by the software ImageJ, as shown in Figure 3c.
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