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One isoform each of PEPCK (XM_003635567.1) and PEPC (XM_002280533.2) were included in the qRT-PCR validation of our RNA-Seq analysis, and it was shown that the expression differences observed between the four developmental stages were consistent across three biological replicates.
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Of the four flight performance measures, only one, take-off angle, gave any indication of differing between the two developmental treatment groups, with developmentally disadvantaged birds tending to take off at shallower angles than their advantaged siblings.
This indicated that the biggest differences exist between these two stages among the four developmental stages.
The expression patterns of transcripts can be divided into two categories by tag copy number among the four developmental stages between two species (Table 2).
These TFs (except of NAC TF) had similar expression profile during the four developmental stages between EG and QS.
Expression patterns were compared at the four developmental stages between QS and EG. Additional file 2: Figure S2 showed the correlation analysis of the ratio values of differential expression level from microarray to that from qRT-PCR.
A MANOVA revealed the presence of a significant difference between the two developmental groups, and follow-up ANOVA's indicated that the desire success and sport awareness subscales contributed to multivariate effect.
As children's language develops at about the same time, some researchers suspected a possible link between the two developmental milestones.
In addition, the number of differentially expressed miRNAs as well as the value of the average fold changes varied between the three developmental ages investigated.
The DIMM expression pattern was basically constant between the two developmental stages, and a simplified overview is illustrated in Figure 2A.
Interestingly, there was no correlation between the capacity to react with the surface of asexual parasite IE and mature sexual parasite IE, indicating that surface antigens might differ significantly between the two developmental stages.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com