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Seventeen hydrogen bonds between the Fab and the receptor ectodomain document the rather polar character of the antibody-receptor interaction (Table 2, Fig. 3A).
The cDNA encoding the Fab can be transferred to an eukaryotic expression plasmid which may also provide a fusion between the Fab and an endoplasmatic reticulum retention signal.
Furthermore, we analysed the correlation between the FAB and EI with different levels of respiratory function, disease duration and depressive symptoms.
Stratified analysis by respiratory function (FVC: ≤80 vs >80) showed a positive correlation between the FAB and EI in low (r=0.559, p<0.001) and high respiratory function subgroups (r=0.731, p<0.001).
Similarly, the analysis by duration of illness (≤18 vs >18 months, median value) showed a positive correlation between the FAB and EI in short (r=0.601, p<0.001) and long disease duration subgroups (r=0.732, p<0.001).
In fact, the correlation between the FAB and other executive tests was present in patients in low and high respiratory function subgroups, assessed in both the early and late stages of disease, and in both depressed and not depressed patients with long disease duration.
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The difference in resolution between the Fab variable and constant regions suggests high flexibility of the elbow angle between these domains (Fig 3B and D).
The residues in JL2 Fab involved in the interactions with SCARB2 are highlighted in bright colors and labeled on the surface (C) Open image in new window Figure 5 The analysis of the detailed interactions between the JL2 Fab and SCARB2.
Five TribodyTM proteins (A1-A5) were generated with different linkers between the Fab-8D3 and scFv-158.
The quantitative analysis presented here identified important differences between the Fab-7 and vg PREs.
However, we also found a striking difference between the Fab-7 and vg PREs at site 3. Whereas the Fab-7 PRE had a PSS score of 1.1 at this site, indicating no PSS) the vg PRE had a PSS score of 71 with homozygous flies displaying completely white eyes.
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