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Sequencedreads were assembled and nucleotide comparisons, single nucleotide polymorphism (SNP), indels were analyzed between the draft genomes of HN016 and YM001.
Whole genome comparisons were performed using the MUMi index [ 30] to assess pairwise distance between the draft genomes and available reference Xanthomonas genomes as shown in the phylogenetic tree and the distance matrix (Additional file 2: Fig. S2).
The sequence identity between the draft genomes ATCC948-1 ATCC948-2 is 98.408.40% after alignment with progressiveMauve (Darling et al. 2010), so most of the analyzed sites for mutation analysis were shared.
Paired alignments between the draft genomes (contigs) and the ORFs from the genomes of CFN42 or CIAT652 were performed using the Dds2 program [ 48], which produces ungapped alignments of fragments having similarities greater than 80%.
To assess regions of genetic similarity between the draft genomes, sequence comparisons were performed with reference to the type strain CD630 and visualized in BRIGv0.95 using BLASTn (Alikhan et al. 2011).
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The average nucleotide identities (ANIs) between the draft genome (P.
Circos provides a visual overview of the alignment between the draft genome and the reference genome.
The average nucleotide identity (ANI) between the draft genome of the non-pathogenic Clavibacter and the three published Clavibacter genomes Cmm NCPPB 382 (NCms09480.1), Cms ATCC 33113 (NC_010407.1) and Cmn NCPPB 2581 (NC_020891.1) was determined using the in silico DNA-DNA hybridization (DDH) method included in the software JSpecies [ 28].
Also, as shown in Additional file 4A, over 78%% of the transcripts lie in the GC range of 35 40 %, while similar levels were reported for its genome (26 40 %) [ 1] as well, indicating a closer molecular signature between the draft genome and the transcriptome of Sf21 cells.
According to these sizes, the numbers of CDSs also varied between the final genomes (1269 1338) and the draft genomes (1258 2321).
All of the draft genomes have ~400 contigs, with an N50 value between 30 and 55 kilobasepairs (Table 1).
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