But at the same time, it is becoming clear that the molecular mechanisms resulting in familial AD pathology differ a lot between the different mutations [ 26].
We did not find any statistically significant difference in the sALP levels between the different mutations groups, which is not surprising since most of patients have already been treated for PDB.
Association between the different mutations was tested using Fisher's exact test.
Also consistent between the two methods is the relative strength of signal between the different mutations, with Myc most easily distinguished by both, and Ras the least distinguishable.
It is obvious that there are epistatic interactions between the different mutations associated with the emergence of drug resistance, the genetic environment, and compensatory mutations, but understanding these complex relationships will require further research.
The HRMA was able to discriminate between the wt DNA and the different mutations present in the mutant cell line DNA.
HRM was able to discriminate between wild-type DNA and the different mutations present in the cell line DNAs using the 189 bp amplicon flanking exon 2. Figure 2 shows a normalised plot and a difference plot of the HRM data using wild-type DNA as a baseline.
To further evaluate the relationships between these different mutations, four additional viruses were constructed in which single and double substitutions at positions E2-60, E2-161 and E2-211 from the Ag41855 strain were substituted into the backbone of LR-GFP-226V (Table 4).
However, the studies published until recently did not differentiate between the different KRAS mutations and other histopathological or molecular features of their patients.
In order to investigate the interactions between organisms with different mutation parameters, the model was modified so that each organism was assigned not only coordinates in the morphospace, but also a distinct value of μ (which was held constant over the entire population in all other simulations).
