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Product recoveries of around 57% were verified during the drying runs; however, the operating parameters were fixed in order to permit quantifiable comparisons between the different feed compositions.
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There was no statistically significant difference for biochemical values between the different feeding groups.
There are more differences in each type of feed than between the different feeds themselves.
Assessing the latter requires additional methods such as direct observations, automated recording systems or video recordings in order to assess for instance time spent feeding and switching frequency between the different experimental feeds.
An interesting observation was the differences in detection levels between different feed and feed ingredients.
Significant differences in the effects of acids on reduction of Salmonella between different feed materials were observed.
The efficacy of acid treatments varied significantly between different feed materials.
To detect any significant differences in microbial taxa (OTU) abundance between the animals with different feeds, we developed a partial statistical model, implemented in custom c++ programs.
Significant variance (p < 0.05) determined by two-way analysis of variance (ANOVA) was applied in between the different parameters of initial feed mixture and final vermiwash.
In spite of the fact that PWD was induced in all challenged groups, many of the parameters measured varied between different feeding regimes, as discussed below.
Cofilin-2 and elf-1α were used as reference genes as they were sufficiently stable across treatments for normalization and had been identified in previous salmon cDNA microarray and qPCR studies as suitable reference genes on the basis of constant expression between different feeds and time points [ 44, 54].
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