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Student's t-test and the Mann-Whitney test were carried out to assay significant differences between the different experimental groups.
Student's t-test, and one-way and two-way ANOVA tests were carried out to assay significant differences between the different experimental groups.
A multivariate analysis (Wilks' Lambda test of multivariate independence) was performed to detect significant differences between the different experimental conditions.
Particularly in experimental designs modulating the load level, analyzing only correct trials that might lead to statistical effects: in the current work, e.g., the number of correct trials decreased with increasing load but this load-related decrease differed between the different experimental groups.
As such, nematode abundances did not differ significantly, neither between the different experimental treatments (enriched) and controls (unenriched), nor between the upper and the lower 5 cm within the colonisation tubes (Figure 2A).
Differences in expression levels between the different experimental conditions (MCF7 control cells, MCF7 hypoxic cells and MCF7 3-D spheroins in the presence or absence of YC-1) were large enough to be detected by semi-quantitative PCR.
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Linear contrasts between regressors, representing the different experimental conditions, were used to determine activated brain areas by generating statistical parametric maps of the t-statistic [SPM t)].
Briefly, before thrombin injection (day 0), the mice received habituation trials for 2 consecutive days (day −2 and day −1), in which the preferences between quadrants in the different experimental groups were eliminated.
To test for changes in connectivity between brain areas under the different experimental conditions, we performed an effective connectivity analysis using DCM (Friston et al. 2003), as implemented in SPM5.
However, differences in behavior between the various strains tested at the different experimental conditions have been detected.
For comparison, the average similarity between the three different experimental set-ups was 26 40% by the end of the experiment.
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