Sentence examples for between the corresponding restriction from inspiring English sources

The amplified ORF were restricted with HindIII and XbaI and cloned between the corresponding restriction sites of the mammalian expression vector pcDNA3.1.

The DNA fragments were cloned between the corresponding restriction sites of plasmid pCN51 in order to place gene transcription under the control of the Pcad promoter.

Mature LytM (without its signal peptide, residues 26 to 316) was overproduced in E. coli using plasmid pAD05, constructed by cloning a 887-bp PCR-generated NcoI/XhoI DNA fragment corresponding to 'lytM (oligonucleotide pair OAD027/OAD018) between the corresponding restriction sites of plasmid pET28/16, replacing the stop codon with an XhoI restriction site.

PCR fragments were cloned between the corresponding restriction sites into the psiCHECK™-2 vector (Promega).

The new plasmids were generated by digesting the binary plasmids G-rk, PX-rk and vac-rk (respectively) with SacI and HindIII, and mobilizing the reporter gene cassettes between the corresponding restriction sites of pBluescript KS.

The sequences are subdivided into libraries, which are labeled with a three-letter code, with the corresponding restriction enzyme listed between brackets.

The pCMMP Gagf vector was constructed by cloning a XhoI-BamHI fragment from pGag-GFP17 into the corresponding restriction sites of pCMMP KRAB18.

First, the AgeI-BamHI KRAB gene fragment from pCMMP KRAB was cloned into the corresponding restriction sites of pCMMP LacZ IRES GFP9, generating pCMMP KRAB IRES GFP.

They were then inserted into the corresponding restriction enzyme sites in Renilla luciferase 3′ UTR of psiCHECK-1 to generate psiCHECK-SM.

The sequences of the restriction sites are underlined and the corresponding restriction enzymes are annotated.

The amplified genes were inserted into the corresponding restriction site of pACYCDuet-1 (Novagen).