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All PCR products were verified on an agarose gel stained with ethidium bromide to discriminate between the correct amplification products and potential primer dimers.
End-point reaction products were analyzed on a 10% polyacrylamide gel stained with ethidium bromide to discriminate between the correct amplification product (57 60 bp) and the potential primer dimmers (<44 bp).
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Positive QPCR amplifications were confirmed as the correct amplification product through direct DNA sequencing.
The RT-PCR products were sequenced to confirm the correct amplification product.
For verification of the correct amplification product, PCRs were analyzed on a 2% agarose gel stained with ethidium bromide.
To validate qRT-PCR purified products were cloned into pMD19-T (Takara) and sequenced to verify the correct target amplification.
In the absence of the minor H antigen allele, control bands are clearly visible, thus demonstrating correct amplification conditions.
Only the primers designed for Mi-csq-1, Mi-eif-3C, Mi-gpd-2, Mi-Y45F10D.4, and Mi-actin genes displayed correct amplification efficiency and specificity.
All PCR fragments were sequenced to ensure correct amplification.
PCR products were sequenced to confirm correct amplification sites.
After the PCR reaction, the correct size of amplification products was verified on an ethidium bromide stained agarose gel.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com