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Neither was any significant difference in reduction capacity observed between the control strain and the APJ1 double deletion mutant.
The maximum difference in OD was 2.5-fold after 48 hours of growth whereas the difference between the control strain and the strain carrying the wtXR was only 1.5 fold.
However, despite the increased nutrient availability no significant differences were observed between the control strain and the FRD1-overexpressing strains TMB3456 and TMB3458 (Additional file 2: Table S4).
To confirm statistically significant variation between the control strain SE02a and the targeted mutants, two-factor ANOVA analyses with replicates were performed for relative OD730 measurements (days 7 – 19) and relative FFA concentration (days 10 – 10).
In the absence of PGM2 overexpression, the combined overexpression of XK, the non-ox PPP and deletion of the GRE3 gene significantly delayed aerobic growth on galactose, whereas no difference was observed between the control strain and the xylose-engineered strain when the PGM2 gene was overexpressed.
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The difference in fluorescence was however less pronounced between the control strains than between the gpd1Δ gpd2Δ strains, which can be explained by the limited redox imbalance in the control strains.
However, body mass did not differ significantly between sedentary and exercised mice of the control strain or between sedentary and exercised mdx mice.
When comparing the uptake of the three main components D-glucose, D-xylose and D-galacturonic acid between the transformants and the control strain, a clear difference is found (Figure 3a and b).
The difference in the β-amyrin levels between the 1057.βA and the control strain appear to be over twice the difference than the best single over-expression achieved (1026.βA).
Mean squared distances (MSD) among the centroids of the sets in the three-dimensional principal components analysis (PCA) projection have been reported to evaluate the distance between the producer and the control strain with the lipid standards for the range 3,050 to 2,800 cm-1.
Mean squared distances (MSD) among the centroids of the sets in the three-dimensional principal components analysis (PCA) projection have been reported to evaluate the distance between the producer and the control strain with the lipid standards for the range 1,500 and 1,350 cm-1.
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