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This later comparison allowed the observation of differences as small as 1 kb between the compared contigs.
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After grouping ESTs as contigs, we used the BLASTN program to compare contig sequences.
The fingerprints of clones in the merged contigs were then analysed manually and compared to contigs assembled based on Southern blot analyses to finalize the contigs [ 31].
Synteny between M. roreri and M. perniciosa was analyzed with the program MUMmer using the NUCmer setting that compares the contigs from both genomes at the nucleotide level.
Furthermore, transcript abundance (microarray intensity) was compared between contigs and singletons using Welch's t-test.
Also, the higher coverage of the plasmid compared with the chromosomal contigs is indicative of a multicopy plasmid, probably between six and seven copies relative to the chromosome.
First, we assembled automatic contigs from the fingerprints using two different contig building strategies and compared the resultant contigs, as described by Wu et al. [ 40].
Assuming that the reference genome is correctly assembled, we compare the contig order and orientation between the predicted assembly and the reference genome.
After finding all possible overlaps between the contigs under consideration, we merge contigs for which there is no overlap ambiguity – that is, if contig a is minimally extended to the right by contig b and then contig c, contigs b and c must also align with each other.
Assembled contigs assigned to the same gene were further compared, and the contig from the best e-value was adopted.
Span includes the estimated lengths of the gaps between the contigs, as well as the contig lengths.
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