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In this study, we analyzed the relationship between the codon usage frequencies of ORFs and their translation rate.
The low correlation mirrors the observed deviations between the codon usage and tRNA concentrations particularly within the low-abundant tRNA set [7].
Alternatively, this finding might be due to the similarity between the codon usage profiles of the donor and host genomes.
There is also significant difference between the codon usage of the genes encoded on the two strands.
We now set out to test whether the coupling found between the codon usage and gene age behaves similarly in human, mouse, and fly.
Additionally, the clustering results of the silkworm superfamily suggest a lack of explicitness in the relationship between the codon usage of mitogenome and species evolution.
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Further, we investigated the correlation between substitution rate and the codon usage of glycolysis genes.
This takes into account the difference in the number of possible non-silent mutations between genes according to the codon usage within genes.
Comparison between the kIspS and the optimized IspS sequence for B. subtilis and B. licheniformis were performed and results showed differences in the codon usage between them as shown in Additional file 5: Table S1 as shaded sequences.
The codon usage between the KPC and Ymf genes in Tetrahymena Mt genomes was extremely similar with very little variation (ANOVA pvalue = 0.9988), however there were three exceptions in Ymf 69, 74, and 71 genes.
To robustly test the statistical significance of our observation for each of the 59 analyzed codons, we measured the variance of the codon usage between the 9 age groups.
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