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However, multiple comparison tests show that only the differences between the coding exons of all four F elements and the coding exons from the base of the D. melanogaster and D. erecta D elements are statistically significant (see File S6).
Our genome analysis also revealed that a large number of the intragenic predictions (which all had three motifs or more within a span of 600 nucleotides) mapped with the introns located between the coding exons.
To determine which of these transcripts are expressed in ESCs, quantitative PCR (Q-PCR) was used to amplify junctions between the coding exons and the alternative 5′ and 3′ untranslated regions (UTRs).
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The coding exons were sequenced in multiple affected family members and compared to the reference sequence.
RESULTS: All the coding exons of OPTN were sequenced in 140 POAG patients and 130 controls.
The coding exons of MamPKR were found in seven contigs that ranged in size between 1 and 50 kb.
Lower panel: The coding exon arrangement of the human aggrecan gene is depicted and the regions of the core protein encoded by each exon is indicated.
These would contain a 5'UTR with at least the untranslated exon -1 and the coding exon 1, but without any of the other coding exons.
To further analyze the difference in the distribution of coding spans and the coding regions between the genes on the F and D elements, we examined the distributions of the number of exons, the coding exon sizes, and intron sizes.
Recombination replaced exon 1, most of exon 2 and the intron between the two coding exons with the neomycin resistance cassette.
The high degree of sequence conservation implies a transfer between the original coding exons, as opposed to a loss of the beginning of the third exon and gain of surrounding genomic sequence at the end of the middle exon.
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