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The values were calculated by the ratio between the cleavage amount and the total radioactivity of each lane, subtracting the control background.
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The cleavage product amount was calculated as the percentage of total input mRNA.
The interval between the cleavage planes is 0.616 nm.
The transition between the cleavage-incompetent and cleavage-competent forms of LexA is associated with a β-turn at the cleavage site A90-G911).
In contrast to a previous report [Wu, H.-N., & Lai, M. M. C. (1989) Science 243, 652-654], we find that chelating Mg2+ with EDTA terminates the cleavage reaction without promoting measurable amounts of ligation of the cleavage products.
The real work of signification is done in the "cleavage between words and meanings".
Unions are hoping that the public-private divide can be trumped by the cleavage between the rich and the rest.
For the excited boys the cleavage between east and west floods their consciousness when the ship passes through the Suez Canal.
But the most ridiculous recent development is two companies who have apparently decided to redesign the human anatomy to remove the cleavage between butt cheeks.
The unedifying clarification was the latest salvo in the "cleavage row" between the paper and a huge Bollywood star, in which it seems that nothing is off-limits.
The amount of CASP3*-LENA and the cleavage pattern of Gag were examined by Western blot (left).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com