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This study clearly showed electrical and morphological coupling between the cell sheet and host myocardium and that the cell sheet could contract synchronously with the beating of the host heart and improve the regional systolic function.
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This study presents a strategy for the initiation of prevascular networks in a 3-D tissue construct by sandwiching endothelial cells between the cell sheets.
Next, they layered single-cell sheets to generate bilayer-cell sheets, forming an electrically communicative three-dimensional cardiac construct, which exhibited spontaneous and synchronous pulsation with electrical communication between the cell sheets, mediated by connexin 43.
Using cell-culture inserts of an appropriate height and the weight of the culture-dish cover, we achieved an appropriately narrow space to facilitate strong adhesion between the cell sheets.
The cell sheet?
The cell sheet from the TNFS only partially detached, allowing for transfer of a partial cell sheet or cell sheet fragments.
In this study we developed a cell sheet using hMSCs as cell source and seeded HUVECs on the cell sheet to form a prevascularized cell sheet, which was further folded to fabricate 3D prevascularized cell sheet constructs.
The cell sheet was fixed in 4% paraformaldehyde overnight.
After spreading cell sheets, the medium was then aspirated to adhere the cell sheet to the culture surface.
The cell sheet has an osteogenic potential and is therefore designated as an osteogenic matrix cell sheet [ 9, 10].
In this study we modified the cell sheet technology based on Okano's cell sheet stacking method.
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