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The FHA domain is linked to the kinase/phosphatase catalytic domain by a flexible tether, and it exhibits a mode of target selection based on electrostatic complementarity between the binding surface and the phosphothreonine peptide.
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In this open conformation, the interactions between the C-terminal μ2 domain and α and σ2 are lost, and the binding surface between the C-terminal domain of μ2 and β is doubled (Jackson et al., 2010).
So the binding surface between PH-D and AC-D is much broader than the specific binding surface between PH-D and the p53 acidic fragments.
We show in Figure 5A C that p53 (Di Lello et al, 2006) and VP16 (Di Lello et al, 2005) use part of the binding surface formed between p62 PH-D and hTFIIEα AC-D.
Some degree of mutual accommodation between the protein binding surfaces and RNA causes the formulation of most protein-RNA complexes.
Although the overall SAXS-based Uch37-Rpn13C comodel model is in agreement with the model reported by Chen et al., the distance between the Uch37-binding surface of Rpn13 and the Uch37 molecule is slightly larger.
The second technique based on backscattered electron is not only limited to gold nanoparticles but also suits for any study of metallic nanoparticles as the electronic density difference between the nanoparticles and binding surface stays high enough.
Additionally, the TAND1 binding surface also overlaps with the binding site of transcriptional activator VP16 resulting in a competition between the two proteins to bind TBP [ 23].
The conformation of the central β-rich region may play an important role in RNA binding by modulating the interaction and presumptive binding surface between the two main domains, which could convey evolutionary advantages and virus specificity.
As such, there is very little overlap between the DH domain binding surfaces of the SH3 domain from Asef and SH3(E) from intersectin-1L, suggesting that these GEFs utilize distinct mechanisms of autoinhibition.
The binding of surface AnxA2 to surface S100A10 also contributes to heterotypic cell-cell interactions between breast tumour cells and microvascular endothelial cells.
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