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Many researches have demonstrated the obvious correlation between the binding degree and pH levels by in vitro assays.
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Therefore, conventional methods are difficult to determine the binding degrees of these components with plasma proteins.
The nonparametric statistical comparison (z-scores) in the table reflects the degree of difference between the binding potential values provided by the methods being compared.
The nonparametric statistical comparison (z-scores) in the table denotes the degree of difference between the binding potential values provided by the methods; *the binding potentials obtained by the two methods differed significantly (two-tailed tests, Bonferroni correction for multiple comparisons, p ≤ 0.0016).
Using the best myogenin dataset, we found a high degree of overlap between the binding sites of the two factors.
We further analyzed the degree of correlation between the binding affinities of probes with different insertion bases X and Y (see Additional file 5): A clear correlation appears between the hybridization signals of probes with T- and G-insertions, and also, though less distinct, between A- and C-insertions.
Still others demonstrated nonspecific binding in that the degree of fluorescence did not significantly differ between the binding of modified and unmodified hASLLys3UUU.
The use of SPR not only overcomes these limitations but also allows the more accurate statistical analysis of the correlation between the degree of binding and drug efficacy.
In all conditions, a clear difference between the degree of binding of core and intact enzymes could be observed, leading to the possibility of recovering the core enzymes, especially the main protein Cel7A after hydrolysis.
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No major differences were observed between the degree of covalent binding in male vs female C57BL/6 mice.
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