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The most obvious explanation for the apparent discrepancy between the binding affinity selectively and the in vivo effects, is the location of adenosine receptors, as A1 receptors are on the endothelium, whereas the A2A receptors are mainly located on vascular smooth muscle [12]; hence the endothelium will be directly exposed to an apparently higher concentration.
Indeed, on-going studies of the relationship between the binding affinity to HLAIs and the structure of LILRA1 will address this hypothesis.
However, additional factors might influence its inhibitory activity explaining cases in which there was no correlation between the binding affinity and the activity of this protein.
We sought to determine whether there was a correlative relationship between the binding affinity and pH thresholds of pore formation in the two receptors.
Although the uptake of FITC-labeled TP5 in EBV-transformed B cells was confirmed, the correlation between the binding affinity of FITC-labeled TP5 to EBV-transformed B cells and the fluorescence intensity in cells was not showed clearly.
This revealed a significant correlation between the binding affinity of epitopes from HIV-1 clade B p24 and the risk of HIV-1 disease progression (Kendall's τ = −0.17, p = 0.029, see Figure 2B).
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Comparison of Kd showed only mild differences between the binding affinities of the wild-type and mutant proteins to histone peptides (Table S1).
The differences between the binding affinities of Pep-7 to HPV16E7 and HPV18E7 were statistically significant (p = 0.004).
Likewise, differences between the binding affinities of Pep-7 and N-pep to HPV16E7 were statistically significant (p = 0.015).
Mint1 MBD was bound at the same molar concentration of syntaxin to allow for a direct comparison between the binding affinities for the two proteins.
The complexes of Src SH2 with peptides of type pYEEX (where X = I,L,V,A,G) have binding affinities correlated with the size of the residue at position pTyr+3, but the differences between the binding affinities are modest, as is also observed in calorimetric studes [9].
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