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We defined the co-culture curve,
There is a linear relationship between the average fluorescence intensities for each inoculum and bacterial CFU.
Mean densitometry was calculated as the ratio between the average fluorescence intensity of the ROI and its area.
In addition, there is a linear relationship between the average fluorescence for each inoculum and the CFU inoculated.
The threshold was set as the midpoint between the average fluorescence amplitude of positives and negative droplet clusters on each of the FAM and VIC channels.
A regression fit was performed on the data to evaluate the correlation between the average fluorescence signal for each inoculum and the CFU levels.
Similar(53)
Relative error of the average fluorescence measurements between day-day repeats is about 10%.
Relative error of the average fluorescence measurements between day-to-day repeats was about 15%.
POP-1 asymmetry was calculated as the ratio of the average fluorescence intensities between the anterior and posterior nuclei quantified using the IP lab software.
To discriminate between Ki-67 negative and positive cells, the average fluorescence intensity of Ki-67 was measured in ciliated cells and nuclei were considered as positive if their Ki-67-associated fluorescence was above this average value.
(B ) Quantification of the average fluorescence intensity ratio between axon region and synaptic region.
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