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In addition, molecular epidemiological typing indicates a relatively close relationship between the analyzed isolates and the previously major prevalent clone identified in different hospital infections due to E. aerogenes [4], [9], [11].
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There was a strong overall match between the clustering based on PFGE dendrogram, spa type, ST, or CC of the analyzed isolates (Table 2; Appendix Figure).
Phylogenetic analysis not only showed a close genetic relationship between the H5N1 strains isolated from poultry and wild migratory waterfowls but also suggested genetic reassortment among the analyzed isolates.
In several instances the nucleotide differences were most likely derived through recombination between futA or futB fragments that were represented by the sequences of the five analyzed isolates.
PFGE and VNTR analysis of analyzed isolates.
The method developed by Anders and Huber [ 36] for analyzing isolate transcriptomes was adapted and used as an additional technique to determine differentially expressed Pfams between communities.
This seems to isolate three main methods of automated maintenance from the analyzed literature.
Recombination between the different isolates was analyzed using the Phi test for recombination implemented in the tool SplitsTree4 [ 56].
The genetic relatedness and clonal relationship between the isolates were analyzed by using the PFGE patterns and the allelic profiles of the sequence types (STs).
Because of little overlap between the isolate sets analyzed by the different typing schemes, ∼90% of the superclusters were composed only of isolates that have been typed by the same method or scheme, and most of the clusters (∼85%) were small (less than five isolates).
We analyzed the isolated LP neurons from 6 different animals.
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