Sentence examples for between the amplified transcript from inspiring English sources

Sequence analysis of the PCR products amplified from an A. nidulans cDNA corresponding to the ORF of the A. nidulans lacB gene identified in this study revealed small differences between the amplified transcript and the predicted ORF deposited in the NCBI database (GenBank gene ID 2876757).

Cleavage points of the amplified transcript were identified using VecScreen http://www.ncbi.nlm.nih.gov/VecScreen/.nih.gov/VecScreen/

All PCR products were subcloned and sequenced to confirm the identity of the amplified transcripts.

Since the amplified transcripts represented a mixture of endogenous and ectopic RNA, we repeated the reverse priming with primers located in the SV40 tail of the UAS constructs.

The comparative threshold cycle or Ct method was used to quantify the amplified transcripts.

Cloning and sequencing of these PCR products confirmed the identity of the amplified transcripts.

The amplified transcripts vary significantly in estimated length, GC content and expression depending on amplification technique.

The amplified transcripts were cloned into a pCR vector (Invitrogen, USA), and the insertion of the transcript was verified by restriction enzyme digestion and sequencing.

As previously mentioned the correlation between unamplified and amplified transcript abundance was intermediate, indicating that both amplification methods have bias and that these biases are different from each other.

The 3' bias of the T7 amplified transcripts helped in the design of primers to discriminate between P. squamulatum and the BC8 pearl millet genome containing one P. squamulatum chromosome.

The genes present on the microarray were divided into two groups according to whether the PCR amplified transcripts (S') or the T7 amplified transcripts (M') were more abundant.