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It can be observed that all the sensors show a linear relationship between the absorbance values and the concentration of ethanol.
It is observed from Fig. 3a, b that a good linearity is obtained between the absorbance values and concentrations of selenite.
Statistical differences between the absorbance values were assessed by the analysis of variance (ANOVA) using GraphPad Prism 4 computer software (San Diego, CA, USA), and significant differences between the means were determined using Dunnett's test [16].
The calculation method is based on the observation that the relationship between the absorbance values of the enzyme immunoassays with and without denaturing washing step is linear.
There was no significant difference between the absorbance values of the MTT assay at 24 and 72 h (ANOVA, p = 0.39).
Secondly, we developed a new calculation method based on our observation from dilution series that the relationship between the absorbance values obtained without and with denaturing agents is linear.
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The extinction coefficient of miRFP670nano was determined as a ratio between the absorbance value of the peak at Q-band and the value of the peak at Soret band, given a Soret band extinction coefficient of 39,900 M−1 cm−116.
This is based on the relationship between the absorbance value at 1,655 cm−1, which corresponds to amide group and the hydroxyl group which is at 3,450 cm−1 (Monteiro and Airoldi 1999).
When comparing the absorbance values between neutralized A and neutralized B solutions, it would be evidenced that the use of saline (NaCl 0.9%) in A solution as an alternative acetic acid diluter increased its compatibility to erythrocyte integrity than commonly used distilled water.
In measurement by ET-AAS, however, non-linearities were obtained between the As (III) concentrations added to milk or yoghurt and the absorbance values.
The absorbance values of ITF2357-treated groups were normalized to the control group at each time point analyzed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com