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Comparison of FAP gene expression measured by real-time PCR between synovial tissue samples taken from patients with end-stage OA or severe RA was performed with Mann-Whitney test (U test).
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The study was entirely carried out on epoxy resin- and paraffin-embedded normal human synovial tissue samples collected at the archives of the Section of Anatomy and Histology, Department of Experimental and Clinical Medicine, University of Florence.
Meniscal and synovial tissue samples were immediately stored at −80 °C.
Synovial tissue samples were obtained by arthroscopy from 62 RA patients before the initiation of infliximab treatment.
Synovial tissue samples for immunohistochemistry were embedded in paraffin.
NKT cells were present in paired synovial fluid and synovial tissue samples.
Finally, we could confirm the correlation between synovial CD21L mRNA and IL-23 mRNA expression in two additional and independent sample sets [r = 0.778, p < 0.0001 in the first confirmation cohort of 36 synovial tissue samples (cohort 2) (Fig. 3e) and r = 0.817, p = 0.011 in the second confirmation cohort of nine synovial tissue samples (cohort 3) (Fig. 3f)].
OA synovial tissue samples contained mostly vascularized connective tissue with spindle-shaped fibroblasts.
Synovial tissue samples from the most actively inflamed and accessible joint were recovered using arthroscopically guided synovial biopsy procedures.
RT-PCR analysis showed JAM-C mRNA expression both in mouse synovial tissue samples and in cultured mouse synovial fibroblasts.
Next we sought to examine the localization of IL-29 expression in RA synovial tissues and performed immunohistochemical analysis on six RA and five normal synovial tissue samples.
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